A Simplified Method for Detecting Fibrinogen and Its Derivatives by Tanned Red Cell HemagglutinationInhibition Immune Assay

Abstract
It is of importance to detect split products derived from fibrinogen or fibrin for the diagnosis and therapy of intravascular proteolysis (digestion of fibrinogen, fibrin, factors V and VIII). A simplified method of detecting the split products was presented by using a tanned red cell hemagglutination inhibition immune assay as in pregnancy test of Pregnosticon or Serotest. The method was easily performed within 2 hours. A minimum sensitivity of fibrinogen concentration to be detected was approximately 5μg per ml. By the application of this test system to gynecological and obstetrical materials, a large amount of the split products was detected in defibrinated plasma or serum samples from the umbilical cord, the uterine cavity during menstruation and patients with obstetrical hypofibrinogenemia.