A re-investigation of the question of activators of carbonic anhydrase

Abstract
Carbonic anhydrase activity was estimated manometrically and by a new colorimetric method which enabled higher enzyme concns. to be used than were previously possible. Some observations were also made on a rapid-flow adaptation of Faurholt''s carbamino method for measuring the rate of hydration of CO2. Provided that all precautions were taken to eliminate errors due to stabilization of the enzyme, no evidence was obtained to support the claims that certain substances are activators of carbonic anhydrase.