Presence of Host-Plasma Membrane Type H+-ATPase in the Membrane Envelope Enclosing the Bacteroids in Soybean Root Nodules
- 1 August 1985
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 78 (4) , 665-672
- https://doi.org/10.1104/pp.78.4.665
Abstract
An improved method is described for the isolation of membrane envelope enclosing the bacteroids (peribacteroid membrane) from soybean (Glycine max L.) root nodules. The ATPase activity of the peribacteroid membrane from infected roots is compared with that of the plasma membrane from uninfected roots. The two ATPases are similar in terms of their vanadate sensitivities, pH optima, and mineral cation requirements, and show antigenic cross-reactivity. However, the ATPase of peribacteroid membrane is more sensitive to stimulation by NH4+. ATP-dependent proton translocation across the peribacteroid membrane was demonstrated in broken protoplasts of infected cells, by the use of fluorescence microscopy with acridine orange. It is suggested that acidification of the peribactroid space by the peribacteroid membrane ATP-ase results in the conversion of NH3 to NH4+ in this space and thereby facilitates the removal of fixed-nitrogen from the bacteroid.This publication has 16 references indexed in Scilit:
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