Rates of Utilization of Glucose in Erythrocytes and Leucocytes

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Abstract
For estimating rates of glycolysis by erythrocytes and leucocytes, in mixtures of the 2 types of cells, heparinized whole blood was centrifuged and divided into two samples, one of red cells with few or no white cells and the other of red cells with many white cells, resuspended in their own plasma. Glycolysis was expressed in [mu]g. of sugar removed/hr./million cells, red and white, in each sample. Separate rates of glycolysis by red and white cells were calculated by solving simultaneous equations derived from the 2 sets of data. In samples of blood from 3 normal dogs and 10 normal human subjects, rates of glycolysis varied from 0.014 to 0.028 [mu]g. of glucose/hr./ million erythrocytes and from 4.7 to 13.2 [][mu]g./hour./million leucocytes. Glycolysis by blood platelets appeared to be insignificant.

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