Stepwise intraclonal maturation of antibody affinity through somatic hypermutation.
- 1 November 1988
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 85 (21) , 8206-8210
- https://doi.org/10.1073/pnas.85.21.8206
Abstract
Using recombinant DNA techniques, we reconstructed a genealogical tree (Sablitzky, F., Wildner, G. and Rajewsky, K. (1985) EMBO J. 4, 345-350) that connects three clonally related B cells producing somatically mutated antibodies to a progenitor cell expressing a germ line-encoded antibody. The somatic mutants had been isolated from an in vivo immune response. The germ line-encoded progenitor antibody bound the antigen with high affinity. Intraclonal affinity maturation occurred stepwise over a 15-fold range.Keywords
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