Abstract
A rapid method was developed for extraction and purification of free abscisic acid (ABA) from leaves of waterlogged seedlings of Juglans. There were significant increases (6-15 fold) in leaf ABA contents of treated plants reaching maximum levels after the first 12 to 18 hours of waterlogging. With the exception of J. nigra, the leaves of which were desiccated at 24 hr after treatment, ABA content rapidly declined to original levels by 30 hours after waterlogging. Changes in ABA concentration in walnut is likely a secondary expression to the stress induced by waterlogging.

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