A Homobifunctional Rhodamine for Labeling Proteins with Defined Orientations of a Fluorophore
- 6 February 1998
- journal article
- Published by American Chemical Society (ACS) in Bioconjugate Chemistry
- Vol. 9 (2) , 160-167
- https://doi.org/10.1021/bc970174e
Abstract
The synthesis and characterization of a bifunctional rhodamine dye bearing 2-(iodoacetamido)ethyl substituents on the 3‘- and 6‘-nitrogen atoms is described. Aspects of the conversion of chloroacetamides to iodoacetamides are discussed, including a remarkably mild dehalogenation of an aromatic haloacetamide in the presence of NaI and camphorsulfonic acid. The bifunctional rhodamine has been designed for two-site, 1:1 labeling of proteins that contain two suitably disposed cysteine residues and is intended to constrain the orientation of the rhodamine absorption and emission dipoles in a predictable relationship to the protein structure.Keywords
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