The Kinetic Spectrophotometric Assay for Serum Alkaline Phosphatase

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Abstract
A rapid and reliable kinetic spectrophotometric procedure for the assay of serum alkaline phosphatase is described. The change in optical density at 400 m[mu] of p-nitrophenyl phosphate was determined in a Beckman DU spectrophotometer equipped with an automatic recording device, a Gilford automatic cuvette positioner and a linear optical density converter. A comparison with the Shinowara-Jones-Reinhart procedure indicates that in the range from 0-50 SJR units of activity the kinetic assay activity is expressed in units approximately 10 times greater. Results on repeated analyses of a pooled specimen gave an average value of 102 units with a standard deviation of 3.2. Sera from healthy adults and from patients with various diseases were assayed.

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