Polymerase Chain Reaction for Definitive Identification of Yersinia Ruckeri
- 1 November 2000
- journal article
- Published by SAGE Publications in Journal of Veterinary Diagnostic Investigation
- Vol. 12 (6) , 558-561
- https://doi.org/10.1177/104063870001200611
Abstract
Yersinia ruckeri causes enteric red mouth (ERM) disease in salmonids. Serologic identification of Y. ruckeri is hampered by cross-reactivity with other bacterial isolates of fish origin. Oligonucleotide primers incorporating Y. ruckeri unique sequences were designed to amplify a 409 bp fragment of Y. ruckeri 16S rDNA. The primers did not amplify other genetically related Yersinia or a wide variety of other aquatic or piscine bacteria. This assay provides a rapid, definitive identification of Y. ruckeri that is not subject to the variability inherent in serologic methods.Keywords
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