Viable deletion mutants in the simian virus 40 early region.

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Abstract
For the purpose of isolating hr-t like mutants of SV-40, variants that have lost the unique site for the restriction enzyme Taq I at 0.565 [tumor]. Five mutants were isolated and characterized by restriction enzyme analysis. All of them produce a normal size T [tumor] antigen. Four produce a T antigen reduced in size and in amount; the 5th one does not seem to make any T antigen at all. The ability of these mutants to transform mouse cells in vitro, as tested by anchorage dependence, is clearly altered; but the defect is only partial. In the same test, the mutants can complement a tsA mutant for transformation and therefore define a 2nd complementation group in the SV-40 early region.