New Vector for Efficient Allelic Replacement in Naturally Nontransformable, Low-GC-Content, Gram-Positive Bacteria
Top Cited Papers
- 1 November 2004
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 70 (11) , 6887-6891
- https://doi.org/10.1128/aem.70.11.6887-6891.2004
Abstract
A shuttle vector designated pMAD was constructed for quickly generating gene inactivation mutants in naturally nontransformable gram-positive bacteria. This vector allows, on X-Gal (5-bromo-4-chloro-3-indolyl-β- d- galactopyranoside) plates, a quick colorimetric blue-white discrimination of bacteria which have lost the plasmid, greatly facilitating clone identification during mutagenesis. The plasmid was used in Staphylococcus aureus , Listeria monocytogenes , and Bacillus cereus to efficiently construct mutants with or without an associated antibiotic resistance gene.Keywords
This publication has 26 references indexed in Scilit:
- clpB, a Novel Member of theListeria monocytogenesCtsR Regulon, Is Involved in Virulence but Not in General Stress ToleranceJournal of Bacteriology, 2004
- SarA and not σB is essential for biofilm development by Staphylococcus aureusMolecular Microbiology, 2003
- Identification of the Up- and Down-Regulated Genes in Vancomycin-Resistant Staphylococcus aureus Strains Mu3 and Mu50 by cDNA Differential Hybridization MethodBiochemical and Biophysical Research Communications, 2000
- A vector for systematic gene inactivation in Bacillus subtilisMicrobiology, 1998
- A general system for generating unlabelled gene replacements in bacterial chromosomesMolecular Genetics and Genomics, 1996
- Overproduction of Encapsulated Insecticidal Crystal Proteins in a Bacillus thuringiensis spoOA MutantNature Biotechnology, 1995
- Use of a new integrational vector to investigate compartment-specific expression of the Bacillus subtilis spollM geneBiochimie, 1992
- Expansion of Insecticidal Host Range of Bacillus Thuringiensis by in vivo Genetic RecombinationBio/Technology, 1992
- Plasmid replication stimulates DNA recombination in Bacillus subtilisJournal of Molecular Biology, 1987
- The toxic shock syndrome exotoxin structural gene is not detectably transmitted by a prophageNature, 1983