Cellular Compartment Analysis of Temporal Activity by Fluorescence In Situ Hybridization (catFISH)
Open Access
- 1 May 2001
- journal article
- unit
- Published by Wiley in Current Protocols in Neuroscience
- Vol. 15 (1) , 1.8.1-1.8.16
- https://doi.org/10.1002/0471142301.ns0108s15
Abstract
This protocol describes a method (cellular compartment analysis of temporal activity by fluorescent in situ hybridization or catFISH) that uses fluorescent in situ hybridization to immediate‐early gene RNAs and confocal microscopy to identify neuronal populations activated at two distinct times. The combination of cellular and temporal resolution makes catFISH a valuable tool for investigating the dynamic interactions of neuronal populations associated with different behaviors or cognitive challenges.Keywords
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