Fibronectin-binding 36 kDa protein in human fibroblasts

Open Access
Publisher Website
Google Scholar
Abstract
A 36 kDa fibronectin‐binding protein was identified from electrophoretically separated proteins of the deoxycholate‐soluble fraction of cultured fibroblasts by blotting with fibronectin and using poly‐ or monoclonal antibodies and immunoperoxidase staining to detect the bound fibronectin. The 36 kDa protein was purified by preparative electrophoresis and used to raise specific antibodies. Solid‐phase 36 kDa protein bound plasma and fibroblast fibronectins equally well. The 36 kDa protein is an amphipathic protein with pI 5.9. It is monomeric with a tendency to dimerize and appears to be distinct from the cell surface fibronectin receptors which interact with the Arg‐Gly‐Asp recognition site in the fibronectin molecule.