Crystal structure of the two RNA binding domains of human hnRNP A1 at 1.75 Å resolution
- 1 March 1997
- journal article
- Published by Springer Nature in Nature Structural & Molecular Biology
- Vol. 4 (3) , 215-222
- https://doi.org/10.1038/nsb0397-215
Abstract
Heterogeneous ribonucleoprotein A1 (hnRNP A1) is an abundant eukaryotic nuclear RNA binding protein. A1 is involved in the packaging of pre-mRNA into hnRNP particles, transport of poly A+ mRNA from the nucleus to the cytoplasm and may modulate splice site selection. The crystal structure of A1(RBD1,2) reveals two independently-folded RNA binding domains (RBDs) connected by a flexible linker. Both RBDs are structurally homologous to the U1A(RBD1), and have their RNA binding platforms oriented in an anti-parallel fashion. The anti-parallel arrangement of the A1 RNA binding platforms suggests mechanisms for RNA condensation and ways of bringing together distant RNA sequences for RNA metabolism such as splicing or transport.Keywords
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