Purification and properties of 2-hydroxy-6-oxo-2,4-heptadienoate hydrolase from two strains of Pseudomonas putida

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Abstract
Growth on phenol of two strains of Pseudomonas putida biotype A, NCIB 10015 and NCIB 9865, elicits the synthesis of an enzyme that hydrolyzes 2-hydroxy-6-oxo-2,4-heptadienoate to 2-oxopent-4-enoate. The purified enzyme from Pseudomonas NCIB 10015 has a molecular weight of 118,000 and dissociates in sodium dodecyl sulfate to a species of molecular weight 27,700; the enzyme from Pseudomonas NCIB 9865 has a molecular weight of 100,000 and dissociates to a species of 25,000 molecular weight. The hydrolases from both strains have similar Km values, pH optima, and thermal labilities and attack the same range of substrates. Neither hydrolase was stimulated by Mg2+ or Mn2+, and both were inhibited by p-chloromercuribenzoate and iodoacetamide. Immunodiffusion studies with the purified enzymes and antibodies formed against them show some cross-reaction of Pseudomonas NCIB 9865 enzymes with antibodies to Pseudomonas NCIB 10015, but not vice versa.