IGE-DEPENDENT ADHERENCE AND CYTO-TOXICITY OF RAT SPLEEN AND PERITONEAL-CELLS TO LITOMOSOIDES-CARINII MICROFILARIAE

  • 1 January 1980
    • journal article
    • research article
    • Vol. 41  (1) , 107-114
Abstract
Serum taken after the termination of microfilaremia from rats infected with the filarial parasite L. carinii brought about adherence and cytotoxicity of normal rat spleen and peritoneal cells to microfilariae. The activity could be absorbed to and eluted from anti-rat Ig[immunoglobulin]E, but not anti-rat IgG immunosorbent columns. Immune serum heated to 56.degree. C for 3 h did not cause cellular adherence or cytotoxicity; the addition of fresh normal rat serum failed to restore activity. Fresh rat serum did restore activity to immune serum which was inactive after being heated to 56.degree. C for 30 min. EDTA, EGTA [ethylene glycol bis(.beta.-aminoethyl ether) tetraacetate] and diethylcarbamazine inhibited adherence. IgE antibodies evidently are responsible for cellular adherence and cytotoxicity and complement may play a part in these reactions.