CLASS OF SERUM ANTIBODIES TOWARDS SALMONELLA ENTERITIDIS IN GASTRO‐ENTERITIS, AS MEASURED BY MIXED REVERSE PASSIVE ANTIGLOBULIN HAEMAGGLUTINATION (MRPAH)

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Abstract
Sera taken from a well-mapped epidemic of S. enteritidis gastroenteritis among school-children and teachers were tested for antibodies of the IgG, IgM and IgA classes by MRPAH (mixed reverse passive antiglobulin hemagglutination) and for IgG and IgM classes by ELISA [enzyme-linked immunosorbent assay]. When suitable threshold titers were chosen, all sera taken up to 10 days after infection were negative by MRPAH; all samples but one taken from day 16-48 showed the presence of antibodies of each 3 immunoglobulin classes. IgG antibody titers often remained elevated 1 yr after infection; IgM and particularly IgA antibodies returned to negative. Up to the 28th day the IgG antibody titer showed a logarithmic increase with time; no simple relationship was observed for the IgM and IgA antibody responses. Estimation of the regression equations for the titers measured by ELISA and MRPAH shows agreement between the 2 methods.