THE CHARACTERIZATION OF SOLUBLE PHOSPHORUS RELEASED BY LIMNETIC ZOOPLANKTON1

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Abstract
Three rapid techniques were used to characterize the main forms of soluble phosphate released by Daphnia rosea. Uniformly 32P‐labeled laboratory animals were transferred to nonradioactive water and most of the soluble radiophosphorus was shown to be similar to PO4 in anion exchange, in gel filtration chromatography, and in a kinetic analysis of incorporation by natural seston. In addition, Diaptomus minutus was labeled in situ with 32P and its release products compared to PO4 by gel filtration. This analysis and those using Daphnia indicated that the released soluble phosphorus was about 90% orthophosphate. However, molybdate analyses of the soluble phosphates released in a 10‐hr experiment showed them to be only 60% orthophosphate. Net reuptake of orthophosphate was insufficient to account for this discrepancy. Recycling of phosphorus is postulated in longer experiments, which leads to a substantial underestimate of the orthophosphate release.

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