Production of Antibodies by Adult Hen Spleen Cells Transferred to Chick Embryos.

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Abstract
Spleen cell suspensions from normal hens inoculated onto chorioallantoic membranes (CAM) with bovine serum albumin (BSA) after in vitro contact with BSA, failed to produce demonstrable antibodies. Whole spleen cell and spleen white cell suspensions, taken from previously normal hens 36 to 48 hours after intravenous injection of BSA and inoculated onto CAM''s, produced significant amounts of antibody without further contact with antigen 4 to 7 days after transfer. The white cell suspensions produced somewhat more antibody than did the whole spleen suspensions. Whole spleen cell suspensions, taken from hens 48 hours after intravenous reinjection of BSA, produced significant amounts of anti-body, without further contact with antigen, 2 to 6 days after transfer to CAM''s. Whole spleen cell suspensions, from hens stimulated by BSA in-jection more than 7 days previously, inoculated onto CAM''s with BSA, or after in vitro contact with BSA, failed to produce demonstrable anti-bodies.