Radioimmunosorbent Assay forBotrytis cinerea

Publisher Website
Google Scholar
Abstract
Radioimmunosorbent assay (RISA) for somatic antigens of the pathogen B. cinerea can be used to detect the presence of Botrytis antigens in homogenized samples. As little as 100 ng of original fungal dry weight can be detected, and the sensitivity curve is log-log linear in response up to 10 mg. The assay is highly specific for B. cinerea although some reaction is obtained with other members of the Sclerotiniaceae. B. allii showed 48% reactivity relative to B. cinerea, and species of Sclerotinia and Monilinia showed 10-24% reactivity. All other fungi tested showed < 0.1% reactivity. The usefulness of the assay for detection of the fungus within host [Vitis vinifera] tissue is demonstrated by the high correlation (r = 0.833) of the assay results with an estimation of rot weight from field-infected lots. Artificially produced infection levels representing 0.1% infected tissue mixed with sound tissue homogenates are easily distinguished from background by the assay. The use of microtiter plates made of polyvinyl chloride greatly increased the response of the assay so that its resolving power was improved.

This publication has 1 reference indexed in Scilit: