Organization of a 117-kb circular mitochondrial chromosome in IR36 rice
- 1 March 1993
- journal article
- research article
- Published by Springer Nature in Current Genetics
- Vol. 23 (3) , 248-254
- https://doi.org/10.1007/bf00351503
Abstract
The organization of the mitochondrial genome in the IR36 indica variety of rice was analyzed using constant-field and CHEF gel electrophoresis. The minimum complexity of the genome is estimated to be 300 kb. There was no evidence for a master circular molecule of this size. The genome exists in vivo in multiple subgenomic circular molecules or mitochondrial chromosomes. Using several rare-cutting restriction enzymes a restriction map was constructed for a 117-kb circular chromosome. Six genetic loci have been placed on this chromosome; cox1 and atp1 have been assigned precise positions while four additional genes (rrn26, cob2, atp6 and rrn18) were mapped to restriction fragments.Keywords
This publication has 36 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- Mitochondrial genome structure of rice suspension culture from cytoplasmic male-sterile line (A-58CMS): reappraisal of the master circleTheoretical and Applied Genetics, 1992
- Plant Mitochondrial Genomes: Organization, Expression and VariationAnnual Review of Plant Biology, 1988
- Separation of Large DNA Molecules by Contour-Clamped Homogeneous Electric FieldsScience, 1986
- Site-specific circularisation at an intragenic sequence in Oenothera mitochondriaMolecular Genetics and Genomics, 1986
- Nucleotide Sequence of ATPase Subunit 6 Gene of Maize MitochondriaPlant Physiology, 1985
- Nucleotide Sequence of the F1-ATPase α Subunit Gene from Maize MitochondriaPlant Physiology, 1985
- Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresisCell, 1984
- Tripartite structure of the Brassica campestris mitochondrial genomeNature, 1984
- Low molecular weight circular and linear DNA in mitochondria from normal and male-sterile Zea mays cytoplasmNature, 1980