Cytogenetic Study of Human Lymphoid T -Cell Lines Derived From Lymphocytic Leukemia 2

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Abstract
Studied were the chromosomes of lymphoid cell lines with characteristics of thymus-derived Iymphocytes (T cells), established from the blood of 1 female patient and 1 male patient with acute lymphoblastic leukemia. The primary blood culture of the female patient had a pseudodiploid mode with characteristic markers; only a few cells were normal diploid. Eight cell lines were established from this patient. Shortly after establishment, all 8 lines had the same predominant karyotype as the primary culture. Several months later, 5 lines lost T-cell characteristics and had characteristics of Iymphocytes derived from the bone marrow (8 cells). At the same time, their karyotype became normal. The 8 cells with diploid karyotype may have represented a selection of normal Iymphocytes that were always present in the patient as well as in the cultures. Two lines were lost in handling. Only 1 line had T-cell characteristics, with a consistent pseudodiploid karyotype, 14 months after establishment. These cells with T -cell markers were probably leukemic. Trypsin and Giemsa banding study revealed the detailed rearrangements and the derivation of the markers in the pseudodiploid karyotype. The cell line from the male patient had a modal chromosome number of 47 at 2-4 months after establishment but a hypertetraploid mode after 11 months in vitro.

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