Highly Efficient Genome Modifications Mediated by CRISPR/Cas9 in Drosophila
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Open Access
- 1 September 2013
- journal article
- research article
- Published by Oxford University Press (OUP) in Genetics
- Vol. 195 (1) , 289-291
- https://doi.org/10.1534/genetics.113.153825
Abstract
We report that Cas9/gRNA mediates efficient genetic modifications in Drosophila. Through targeting seven loci, we achieved a germline efficiency of up to 100%. Genes in both heterochromatin and euchromatin can be modified efficiently. Thus the Cas9/gRNA system is an attractive tool for rapid disruption of essentially any gene in Drosophila.Keywords
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