Structural and genetic analyses of HLA class I molecules using monoclonal xenoantibodies
- 1 August 1983
- journal article
- research article
- Published by Wiley in Tissue Antigens
- Vol. 22 (2) , 107-117
- https://doi.org/10.1111/j.1399-0039.1983.tb01176.x
Abstract
The monoclonal antibodies B1.23.2 and B9.12 were developed to characterize human class I major histocompatibility gene products. They detect 2 different epitopes and define on a given lymphoblastoid B cell line 2 subsets of .beta.2microglobulin[.beta.2m]-complexed HLA class I molecules. One subset reacts with B9.12 and B1.23.2 and the other one expresses only the B9.12 epitope. Binding assays were performed on C3H mouse L cells which were transformed with various single HLA-class I genes and the 2 detected molecular subsets were shown to be encoded by different genetic loci. Unlike B1.23.2, B9.12 detects all the .beta.2m-complexed molecules expressed in human B cell lines and recognizes an epitope different from the one defined by W6/32. In contrast to the vast majority of the other reported anti-class I monoclonal antibodies (including B9.12), B1.23.2 recognizes an epitope expressed on both .beta.2m-associated and -free HLA class I H chains.This publication has 38 references indexed in Scilit:
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