Recognition and Laboratory Characteristics of an Atypical Oocyst of Cryptosporidium

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Abstract
Feces from some patients with clinically unremarkable cryptosporidiosis contained an unusual variety of oocyst not previously recognized. These atypical oocysts were shown by electron microscopy to have a distinctive three-layered outer coat and, by immunofluorescence with a monoclonal antiserum, to lack an antigen present on the surface of typical oocysts. In contrast to typical oocysts, the atypical variety is very fragile and quickly collapses when suspended in solutions of high osmotic pressure or in lipid solvents. Atypical oocysts cannot be stained by methods used to stain typical oocysts, but their appearance in sucrose-phenol is characteristic. Their stability in this solution, though much less than that of typical oocysts, is sufficient for them to be recognized and for cases to be diagnosed by microscopy. Patients who excreted atypical oocysts never excreted the typical variety. General findings in patients who excreted atypical oocysts were no different from those who excreted typical oocysts.