FACTORS AFFECTING ERYTHROID COLONY GROWTH (CFU-E) FROM HUMAN MARROW

  • 1 January 1977
    • journal article
    • research article
    • Vol. 5  (4) , 250-253
Abstract
Human marrow grown in a plasma clot gives rise to discrete colonies of nucleated red cells in response to erythropoietin (EPO). In 10 studies of human marrow with normal erythropoiesis, human EPO in doses up 10 1 unit/ml produced a significant increase in colonies when compared to sheep EPO. To achieve 50% of maximum colony growth, only 0.1 units ml of human EPO was needed in contrast to 0.5 units/ml of sheep EPO. In 7 other experiments, nandrolone decanoate, 10-7 to 10-9 M, yielded a mean increase in colonies of 38% when compared to cultures without added androgen. This effect was entirely dependent on the presence of EPO. The method is useful for the in vitro study of factors affecting early events in erythropoiesis.

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