The Growth Substances separated from Plant Extracts by Chromatography

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Abstract
1. A method for the running of ‘strip’ chromatograms of plant extracts, as large-scale sources of the naturally occurring growth substances accelerator α (α) and inhibitor β (β), and the elution of these substances together with indole-3-acetic acid (IAA), is described. A method is given for the testing of the pea root section extension properties of these growth substances. 2. Coleoptile and root section extension tests over a complete concentration range are done for α, β, and eluted IAA, and mixtures of α and β with IAA or indole-3-acetonitrile (IAN) are tested for coleoptile section extension. 3. α promotes at low concentrations and inhibits at high concentrations both coleoptile and root section extension and the coleoptile section extension induced by IAA or IAN. β inhibits coleoptile and root section extension over the whole concentration range; it also inhibits IAA and IAN induced coleoptile section extension. 4. The extension of coleoptile sections in mixtures of α or β with IAA is measured at a number of time intervals. α, alone and with IAA, has its greatest promoting effect in the early stages and its greatest inhibiting effect in the later stages of section growth. β, alone, promotes the early stages and inhibits the later stages of section growth and, with IAA, has its greatest inhibitory effects in the later stages.

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