Preparation of poly (A)-binding proteins from endoplasmic reticulum-containing subfractions of RAT liver cells and their use in mRNA purification
- 1 March 1977
- journal article
- letter
- Published by Springer Nature in Molecular and Cellular Biochemistry
- Vol. 15 (1) , 67-71
- https://doi.org/10.1007/bf01731290
Abstract
Approximately 2% of the proteins solubilised from rat liver microsomes or rapidly sedimenting endoplasmic reticulum (RS-ER) adsorbed to poly(A)-Sepharose. The adsorption appeared to be selective for a few proteins, and proteins of different apparent molecular weights adsorbed from RS-ER and the microsomes. The proteins from RS-ER with affinity for poly(A) were coupled to Sepharose and used for the purification of mRNA from rabbit mammary glands. A portion of the RNA which did not adsorb to poly(U)-Sepharose adsorbed to protein-Sepharose and was active in a cell-free protein synthesis system.This publication has 14 references indexed in Scilit:
- Membrane-bound polyribosomes in HeLa cells: Association of polyadenylic acid with membranesJournal of Molecular Biology, 1974
- Mammalian proteins with affinity to polynucleotides: Isolation by affinity chromatography from rat liver cytosol and nucleosolFEBS Letters, 1974
- Characterization of a Protein Species Isolated from HeLa Cell Cytoplasm by Affinity Chromatography on Polyadenylate-SepharoseProceedings of the National Academy of Sciences, 1974
- A rapidly sedimenting fraction of rat liver endoplasmic reticulumJournal of Cell Science, 1973
- Efficient Translation of Tobacco Mosaic Virus RNA and Rabbit Globin 9S RNA in a Cell-Free System from Commercial Wheat GermProceedings of the National Academy of Sciences, 1973
- A Protein of Molecular Weight 78,000 Bound to the Polyadenylate Region of Eukaryotic Messenger RNAsProceedings of the National Academy of Sciences, 1973
- Isolation of mRNA from KB‐Cells by Affinity Chromatography on Polyuridylic Acid Covalently Linked to SepharoseEuropean Journal of Biochemistry, 1972
- Preparation of sepharose-bound poly (rI:rC)Biochemical and Biophysical Research Communications, 1971
- An Adenylic Acid-Rich Sequence in Messenger RNA of HeLa Cells and Its Possible Relationship to Reiterated Sites in DNAProceedings of the National Academy of Sciences, 1971
- ISOLATION AND CHARACTERIZATION OF RIBOSOMAL RIBONUCLEIC ACIDBiochemical Journal, 1965