IDENTIFICATION OFCORYNEBACTERIUM DIPHTHERIAEWITH FLUORESCENT ANTIBACTERIAL REAGENTS

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Abstract
Moody, MaxD. (Communicable Disease Center, Atlanta, Ga.)and Wallis L. Jones. Identification ofCorynebacterium diphtheriaewith fluorescent antibacterial reagents. J. Bacteriol.86:285–293. 1963.—Conditions are described whereby fluorescent-antibody reagents could be prepared and used to identifyCorynebacterium diphtheriaein pure and mixed cultures and in clinical materials. The use of O and OK antigens for immunization of rabbits to prepare the antibody was compared. The most satisfactory reagents were those made from serum of rabbits injected with live (OK) suspensions ofC. diphtheriae. Such fluorescent reagents were used successfully in direct and indirect fluorescent-antibody tests to identify both toxinogenic and atoxinogenicC. diphtheriaebut not to differentiate the two kinds of organisms.