Effect of the intracellular localization of a Gd‐based imaging probe on the relaxation enhancement of water protons
Top Cited Papers
Open Access
- 31 January 2006
- journal article
- research article
- Published by Wiley in Magnetic Resonance in Medicine
- Vol. 55 (3) , 491-497
- https://doi.org/10.1002/mrm.20793
Abstract
Gd-HPDO3A has been internalized into rat hepatocarcinoma cells in the cytoplasm (by electroporation) or in intracellular vesicles (by pinocytosis), respectively. In the former case, the observed relaxation rates are likely dependent upon the amount of internalized paramagnetic complex, whereas in the latter case the relaxation enhancement is “quenched” to a plateau value (about 3 s−1) when the entrapped amount of Gd-chelate is higher than 1 × 1010 Gd/cell. The observed behavior has been accounted in terms of a theoretical treatment based on equations formally derived by Labadie et al. (J Magn Reson B 1994;105:99–102). On this basis, entrapment into intracellular vesicles has been treated as a three-site water exchange (extracellular/cytoplasm/vesicle compartments), whereas the cell pellets containing the paramagnetic agent spread out in the cytoplasm can be analyzed by a two-site exchange system. Magn Reson Med, 2006.Keywords
This publication has 22 references indexed in Scilit:
- Magnetic resonance imaging visualization of targeted cells by the internalization of supramolecular adducts formed between avidin and biotinylated Gd3+ chelatesJBIC Journal of Biological Inorganic Chemistry, 2004
- In vivo cellular imaging of magnetically labeled hybridomas in the spleen with a 1.5-T clinical MRI systemMagnetic Resonance in Medicine, 2004
- Improved route for the visualization of stem cells labeled with a Gd‐/Eu‐Chelate as dual (MRI and fluorescence) agentMagnetic Resonance in Medicine, 2004
- Highly efficient endosomal labeling of progenitor and stem cells with large magnetic particles allows magnetic resonance imaging of single cellsBlood, 2003
- Electroporation: theory and methods, perspectives for drug delivery, gene therapy and researchActa Physiologica Scandinavica, 2003
- Cell internalization of anionic maghemite nanoparticles: Quantitative effect on magnetic resonance imagingMagnetic Resonance in Medicine, 2003
- Insights into the use of paramagnetic Gd(III) complexes in MR‐molecular imaging investigationsJournal of Magnetic Resonance Imaging, 2002
- Interaction of Anionic Superparamagnetic Nanoparticles with Cells: Kinetic Analyses of Membrane Adsorption and Subsequent InternalizationLangmuir, 2002
- Hepatocyte Targeting with Gd-EOB-DTPAInvestigative Radiology, 2001
- Fundamentals of electroporative delivery of drugs and genesBioelectrochemistry and Bioenergetics, 1999