An Improved Laboratory Method for Testing Materials as Screw-Worm Larvicides1

Abstract
The medium in which the larvae are reared and tested consists of: Citrated beef blood 15%, water 44.7%, ground lean meat 40%, and formalin (37% formaldehyde) 0.3%. The insecticide is incorporated in the medium from an acetone-Tween-20 solution. The toxicity of the materials is determined by adding newly hatched screw-worm larvae to the medium containing the insecticide and recording knock-down and mortality of the larvae over a period of 48 hours.

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