On the Revivification of Certain Species of Protozoa after Twenty Years of Encystment

Publisher Website
Google Scholar
Abstract
A protozoan culture from an old watering trough was allowed to dry up, after a census of the species had been made. The dried sediment was placed in a piece of glass tubing 123 mm. long after one end had been sealed in a Bunsen flame. The sediment was shaken down into the sealed end after the end had co.oled. This end, with its contained sediment, was kept cool, and the other end of the tubing was sealed in the Bunsen flame. The tube was kept thus for 20 yrs. It was then opened, and its contained sediment cultured with great precaution against introduction of any living forms. Oikomonas sp. appeared in the culture, and also a Mastigamoeba sp. Mastigamoeba had been listed in the census of the culture 20 yrs. before, also "several different forms of minute Mastigophora." It is concluded that, in view of the pre-cautions taken during the experiment, the species which appeared in the new culture, did so after 20 yrs. of encystment. (Full details of the methods of culturing, and the precautions taken, and the checks run at the same time, appear in the paper.).

This publication has 1 reference indexed in Scilit: