Methylation of tRNA Asp by the DNA Methyltransferase Homolog Dnmt2
Top Cited Papers
- 20 January 2006
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 311 (5759) , 395-398
- https://doi.org/10.1126/science.1120976
Abstract
The sequence and the structure of DNA methyltransferase-2 (Dnmt2) bear close affinities to authentic DNA cytosine methyltransferases. A combined genetic and biochemical approach revealed that human DNMT2 did not methylate DNA but instead methylated a small RNA; mass spectrometry showed that this RNA is aspartic acid transfer RNA (tRNA Asp ) and that DNMT2 specifically methylated cytosine 38 in the anticodon loop. The function of DNMT2 is highly conserved, and human DNMT2 protein restored methylation in vitro to tRNA Asp from Dnmt2-deficient strains of mouse, Arabidopsis thaliana, and Drosophila melanogaster in a manner that was dependent on preexisting patterns of modified nucleosides. Indirect sequence recognition is also a feature of eukaryotic DNA methyltransferases, which may have arisen from a Dnmt2-like RNA methyltransferase.Keywords
This publication has 21 references indexed in Scilit:
- EUKARYOTIC CYTOSINE METHYLTRANSFERASESAnnual Review of Biochemistry, 2005
- Genome-Wide Insertional Mutagenesis of Arabidopsis thalianaScience, 2003
- Dnmt3a and Dnmt3b Are Transcriptional Repressors That Exhibit Unique Localization Properties to HeterochromatinJournal of Biological Chemistry, 2001
- Structure of human DNMT2, an enigmatic DNA methyltransferase homolog that displays denaturant-resistant binding to DNANucleic Acids Research, 2001
- Dnmt2 is not required for de novo and maintenance methylation of viral DNA in embryonic stem cellsNucleic Acids Research, 1998
- A candidate mammalian DNA methyltransferase related to pmt1p of fission yeastHuman Molecular Genetics, 1998
- The fission yeast gene pmt1+encodes a DNA methyltransferase homologueNucleic Acids Research, 1995
- A targeting sequence directs DNA methyltransferase to sites of DNA replication in mammalian nucleiPublished by Elsevier ,1992
- Predictive motifs derived from cytosine methyltransferasesNucleic Acids Research, 1989
- On the mechanism of inhibition of DNA-cytosine methyltransferases by cytosine analogsCell, 1983