Diagnosis of Enterovirus Infection by Genus-Specific PCR and Enzyme-Linked Immunosorbent Assays
Open Access
- 1 February 2003
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 41 (2) , 841-844
- https://doi.org/10.1128/jcm.41.2.841-844.2003
Abstract
PCR for the diagnosis of enterovirus infections is resource intensive but is increasingly used due to wide availability. Enzyme-linked immunosorbent assays (ELISAs) that detect heterotypical antibodies against enterovirus immunoglobulin M (IgM), IgA, and IgG were compared with reverse transcription-PCR by using primers specific to the 5′ untranslated regions of 60 enterovirus species. The ELISAs were less sensitive than the PCR, and only the ELISA for IgM was highly specific. When retrospective diagnosis is important or when specimens are unsuitable for PCR, the ELISA has a limited role if PCR is not available.Keywords
This publication has 29 references indexed in Scilit:
- Antiviral agents for influenza, hepatitis C and herpesvirus, enterovirus and rhinovirus infectionsThe Medical Journal of Australia, 2001
- Enterovirus antibody levels during the first two years of life in prediabetic autoantibody-positive childrenDiabetologia, 2001
- Neurological Manifestations of Enterovirus 71 Infection in Children during an Outbreak of Hand, Foot, and Mouth Disease in Western AustraliaClinical Infectious Diseases, 2001
- ‘Original antigenic sin’ phenomenon in neutralizing antibody responses in children with enterovirus meningitisJournal of Clinical Virology, 2000
- Enterovirus infection as a risk factor for beta-cell autoimmunity in a prospectively observed birth cohort: the Finnish Diabetes Prediction and Prevention Study.Diabetes, 2000
- Letters to the EditorJournal of Paediatrics and Child Health, 1999
- Diagnosis of enteroviral central nervous system infection by polymerase chain reaction during a large community outbreakThe Pediatric Infectious Disease Journal, 1994
- Serum IgA, IgG, and IgM responses to different enteroviruses as measured by a coxsackie B5‐based indirect ELISAJournal of Medical Virology, 1992
- Avoiding false positives with PCRNature, 1989
- COXSACKIE-B-VIRUS-SPECIFIC IgM RESPONSES IN CHILDREN WITH INSULIN-DEPENDENT (JUVENILE-ONSET; TYPE I) DIABETES MELLITUSThe Lancet, 1983