Subcellular distribution of ubiquitin–protein conjugates in the hippocampus following transient ischemia

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Abstract
Ubiquitin–protein conjugates in the hippocampus were analyzed by immunoblotting with a monoclonal anti‐ubiquitin antibody. In the CA1 region, Triton X‐100 insoluble ubiquitin–protein conjugates increased after 24 hr following 20 min of ischemia. When the total hippocampi were fractionated subcellularly, ubiquitin–protein conjugates increased in the particulate, especially in the mitochondrial fraction. The ubiquitin–protein conjugates were solubilized by SDS, or were partially solubilized by urea. The results indicate that insoluble ubiquitin–protein conjugates increase after ischemia.