Proapoptotic activity of ITM2Bs, a BH3-only protein induced upon IL-2-deprivation which interacts with Bcl-2
- 7 May 2002
- journal article
- research article
- Published by Springer Nature in Oncogene
- Vol. 21 (20) , 3181-3189
- https://doi.org/10.1038/sj.onc.1205464
Abstract
Growth factor deprivation is a physiological mechanism to induce apoptosis. We used an IL-2-dependent murine T cell line to identify proteins that trigger apoptosis. Here we report the identification, the cloning and characterization of ITM2B(s), a protein induced upon IL-2-deprivation. ITM2B(s), which shares the BH3 domain of Bcl-2 family members, is a cytoplasmic and mitochondrial protein. Expression of ITM2B, induces apoptosis in IL-2-stimulated cells, but not in IL-4-stimulated cells, while overexpression of the long form of the protein is not able to induce apoptosis. In IL-2-stimulated cells, ITM2B(s) interacts with the antiapoptotic protein Bcl-2, and does not interact with the proapoptotic Bad. Mutation of the critical L and D residues within the BH3 domain abolished the ability of ITMBs, to promote apoptosisKeywords
This publication has 28 references indexed in Scilit:
- Noxa, a BH3-Only Member of the Bcl-2 Family and Candidate Mediator of p53-Induced ApoptosisScience, 2000
- IL-2 deprivation triggers apoptosis which is mediated by c-Jun N-terminal kinase 1 activation and prevented by Bcl-2Cell Death & Differentiation, 1999
- The Bcl-2 Protein Family: Arbiters of Cell SurvivalScience, 1998
- Interleukin-3-Induced Phosphorylation of BAD Through the Protein Kinase AktScience, 1997
- Akt Phosphorylation of BAD Couples Survival Signals to the Cell-Intrinsic Death MachineryCell, 1997
- Prevention of Apoptosis by Bcl-2: Release of Cytochrome c from Mitochondria BlockedScience, 1997
- Bax Can Antagonize Bcl-XL during Etoposide and Cisplatin-induced Cell Death Independently of Its Heterodimerization with Bcl-XLJournal of Biological Chemistry, 1996
- Isolation of Markers for Chondro-osteogenic Differentiation Using cDNA Library SubtractionPublished by Elsevier ,1996
- bcl-x, a bcl-2-related gene that functions as a dominant regulator of apoptotic cell deathCell, 1993
- High-affinity and intermediate-affinity forms of the human interleukin 2 receptor, expressed in an interleukin 9-dependent murine T cell line, deliver proliferative signals via differences in their transduction pathwaysCytokine, 1993