ENZYMATIC MICROASSAY OF PYRIDOXAL-5'-PHOSPHATE USING L-TYROSINE APODECARBOXYLASE AND L-(1-C-14) TYROSINE

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  • 1 January 1976
    • journal article
    • research article
    • Vol. 46  (2) , 160-164
Abstract
The method is based upon the stoichiometric decarboxylation of L-(1-14C) tyrosine by L-tyrosine apodecarboxylase and pyridoxal-5''-phosphate [PLP]. The main features of this method are the ease of preparation of the enzyme extract and the substrate, elimination of the preincubation period and shortening of the 14CO2-trapping step. This assay is rapid, highly sensitive and specific.