Properties and Structure of Lipoproteins.

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Abstract
The extraction of human serum lipoproteins of density less than 1.063 g/ml with ether produces two distinct ultracentrifugal components. Lipoproteins of density less than 1.006 g/ml prepared by centrifuging human serum at 30,000 rpm for 3 hours reveals only one ultracentrifuge component following ether extraction. This suggests that the basic protein moiety of lipoproteins of density 1.006-1.063 g/ml may be different from that associated with lower density lipoproteins. Extraction of lipoproteins of density less than 1.063 g/ml prepared from essential hyperlipemic human serum, and hypercholesterolemic rabbit serum reveals a marked predominance of the slower moving component associated with the lower density lipoproteins. These findings suggest that both components may be necessary in transformation of lower density lipoproteins to higher density ones. Absence of the higher density moiety might result in an accumulation of lipid in lower density lipoproteins as occurred in the case of essential hyperlipemia and lipemic rabbit serum. Incubation of serum with radioactive cholesterol followed by paper electrophoresis demonstrates that both alpha and beta lipoproteins bind cholesterol in vitro. This finding suggests the possibility that serum cholesterol levels may partially depend upon this mechanism of combination.

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