RESPIRATORY SYNCYTIAL VIRUS .1. CONCENTRATION AND PURIFICATION OF INFECTIOUS VIRUS

  • 1 January 1978
    • journal article
    • research article
    • Vol. 32  (4) , 265-272
Abstract
Respiratory syncytial (RS) virus can be purified without losing its infectivity provided that each step of purification is carried out using NT buffer containing over 20% sucrose. The virus grown on [human embryonic skin] HES cells is efficiently removed from the culture fluid by precipitating with polyethylene glycol (PEG) 6000, and the precipitate is suspended in a small amount of 20% sucrose-NT buffer, which results in about a 24-fold concentration of the original material. Then this suspension is centrifuged through 30% sucrose-NT buffer to obtain pellets, which are again suspended in 20% sucrose-NT buffer. This suspension is further centrifuged by discontinuous and linear sucrose density gradient. Finally, the specific infectivity of the purified virus was increased about 3000-fold over that of the original material.

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