INCORPORATION OF RADIOIRON INTO MYOGLOBIN IN AN IN VITRO SYSTEM*

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Abstract
Radioiron was incorporated into the heme of myoglobin in the supernatant solutions from dog muscle homogenates. Protopor-phyrin and S-aminolevulinic acid augmented the incorporation of radioiron. Cysteine and reduced glutathione were also found to increase radioiron uptake. Glycine plus succinate augmented radioiron uptake in muscle brei. Heat, KCN and parachloromercuriphenylsulfonic acid inhibited this process. The conditions which favor Fe incorporation into myoglobin heme closely resemble those known to favor heme biosynthesis in other tissues. Preliminary observations suggest that extracts from both normal and dystrophic human muscle are capable of carrying out this process.