Age‐related analysis of EcoRI generated satellite DNA‐containing chromatin of rat liver

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Abstract
EcoRI digestion of nuclei and their subsequent lysis with EDTA solubilizes 45% and 36% of chromatin DNA from the liver of young (18±2 weeks) and old (100±5 weeks) rats, respectively. After hybridization with 185 bp rat satellite I DNA, these soluble fractions are found to be enriched in specific DNA sequences such as satellite DNA. Besides regular repeat pattern, a major portion of the satellite chromatin forms higher order organization. Digestion kinetics confirms condensation of satellite DNA‐containing chromatin similar to that of bulk chromatin in old age. Furthermore, densitometric scanning of the slot‐blot of soluble chromatin fractions reveals loss of satellite DNA in the old. However, an increase in the linker histone H1 and its subfraction H10 in the satellite DNA‐enriched fraction of chromatin from old rats suggests greater compaction. These results provide the first evidence that the satellite DNA‐containing chromatin differs in the liver of young and old rats.

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