An Insect Cell Line Persistently Infected with a Baculovirus-Like Particle

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Abstract
A persistent infection by a baculovirus-like particle was found in the established lepidopteran (Heliothis zea) cell line, IMC-HZ-1. The virus caused CPE [cytopathic effect] in less than 1% of the IMC-HZ-1 cells, as measured by phase-contrast and EM. Transmission tests showed that 4 lepidopteran cell lines were susceptible to the persistent virus (designated as IMC-HZ-1-NOV). In inoculated TN-368 cell cultures, 90-100% infection was achieved. The ultrastructure and development of IMC-HZ-1-NOV in cell cultures were similar to known baculoviruses, and it is probable that this persistent virus is a member of the family Baculoviridae. Two lepidopterous species (Estigmene acrea and H. zea) inoculated with IMC-HZ-1-NOV by intrahemocoelic injection and/or per os feeding of larvae were not susceptible.

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