INFECTIVITY OF ANCYLOSTOMA CANINUM LARVAE AFTER FREEZING OVER LIQUID NITROGEN
- 10 March 1976
- journal article
- Published by Wiley in Australian Veterinary Journal
- Vol. 52 (3) , 141-143
- https://doi.org/10.1111/j.1751-0813.1976.tb05449.x
Abstract
Third-stage larvae of Ancyclostoma caninum were exsheathed either by carbon dioxide or sodium hypochlorite treatment. Exsheathment per se reduced the infectivity of third-stage larvae by 45% to 59% without affecting larval motility. Both ensheathed and exsheathed larvae were frozen over liquid nitrogen for 90 days, and thawed. Frozen ensheathed larvae were all killed; but frozen exsheathed larvae were 40-54% as infective as non-frozen exsheathed larvae, and the addition of 10% dimethyl sulphoxide raised the infectivity to 89%, relative to non-frozen exsheathed larvae. Taken together, the deleterious effects of exsheathment and freezing resulted in an infectivity of 16-37%, relative to normal larvae.Keywords
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