Characterization of a Murine IgG3 Monoclonal Antibody against Chlamydia‐Specific Lipopolysaccharide and its Purification by Affinity Chromatography on IgG Fc‐Receptors from Streptococcus Dysgalactiae
- 12 January 1991
- journal article
- Published by Wiley in Journal of Veterinary Medicine, Series B
- Vol. 38 (1-10) , 11-16
- https://doi.org/10.1111/j.1439-0450.1991.tb00839.x
Abstract
Summary: Purification of a murine IgG3 monoclonal antibody (Mab 22) directed against an epitope of Chlamydia‐specific lipopolysaccharide by affinity chromatography on recently described solid phase IgG Fc‐receptors from Streptococcus dysgalactiae is reported. SDS‐PAGE studies revealed the purity of the eluted antibody. The purified Mab 22 was characterized by determination of class, subclass and light chain‐type, and by dot tests and immunoblot analysis.Keywords
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