Effects of D-Limonene and α-D-Pinene on in Vitro Carbohydrate Dissimilation and Methane Formation by Rumen Bacteria

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Abstract
The effects of D-limonene and a-D-pinene on the in vitro dissimilation of carbohydrates and formation of methane by rumen bacteria were studied by enrichment culture techniques. D-Limonene (9 X 10-4 [image]), but not 9 X 10-4 [image] [alpha]-D-pinene, was toxic to stabilized rumen cultures which produced methane from acetic acid. Methane production from formic acid by rumen cultures was not affected by these terpenes. Cellulose digestion, but not starch and cellobiose dissimilation, by rumen bacteria was inhibited by higher concentrations of both terpenes.
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