Different Responses of Apolipoprotein A-I, A-IV, and B Gene Expression during Intestinal Adaptation to a Massive Small Bowel Resection in Rats

1 January 1997

journal article
Published by Taylor & Francis in Bioscience, Biotechnology, and Biochemistry

Vol. 61 (11) , 1810-1813
https://doi.org/10.1271/bbb.61.1810

Abstract

Gene expression of apolipoproteins (apo) A-I, A-IV, and B, the predominant protein components of chylomicrons, was investigated in the residual ileum after a massive small bowel resection in rats. A Northern blot analysis showed that the apo A-IV mRNA level, but not the apo A-I and B mRNA levels, in the ileum was significantly higher in the resected rats than in the sham-operated rats 24 h and 2wk post-surgery. RT-PCR coupled with a primer extension assay revealed that the apo B-48 mRNA/apo B-100 mRNA ratio, i.e., apo B mRNA editing, in the ielum was unchanged by the resection. It is thus concluded that, among the major intestinal apolipoproteins, apo A-IV is the only one whose gene expression is influenced by loss of the proximal intestine.

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