Human fat cell adenylate cyclase: regional differences in adrenaline responsiveness

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Abstract
The effect of adrenaline on adenylate cyclase activity in membranes prepared from human abdominal and gluteal adipose tissue was examined. Basal enzyme activity averaged 1.0 nmol cAMP/mg protein/15 min in abdominal tissue and 0.68 nmol cAMP/mg protein/15 min in ghosts from the buttock. Maximally effective concentrations of sodium fluoride (20 mmol/l) induced an eight‐fold increase in both membrane preparations. The maximal response to adrenaline (1.0 mmol/l) averaged 170% in ghosts of abdominal tissue compared with 60% in gluteal membranes. The concentrations of adrenaline required to produce half‐maximal stimulation were similar in membrane preparations of both regions (about 5 × 10‐5 mol/l).