The Excision of N‐Methyl‐N‐nitrosourea‐Induced Lesions from the DNA of Chinese Hamster Cells as Measured by the Loss of Sites Sensitive to an Enzyme Extract that Excises 3‐Methylpurines but not O6‐Methylguanine

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Abstract
An enzyme extract containing glycosylase from Micrococcus luteus excises 3-methyladenine and 3-methylguanine but not O6-methylguanine, 7-methylguanine, 1-methyladenine or 7-methyladenine from DNA reacted with N-methyl-N-nitrosourea. The extract was used to detect lesions in the DNA of Chinese hamster cells treated in culture with N-methyl-N-nitrosourea. Methyladenine is excised from these cells with a half-life of .apprx. 2.3 h.