Rapid Alkaline Rehydration of Dried Plant Tissues for Histologic Study

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Abstract
Pieces of leaf tissue were soaked in 3% aqueous KOH at about 75 C for 20-30 min, washed, evacuated, dehydrated, embedded in paraffin, and 5-7 μ sections attached to slides with gelatin adhesive. Compared with fresh-fixed control material, dried material stained with less contrast, but the general morphology of cells and tissues was fully restored. Nuclei and chloroplasts were preserved only occasionally, but such delicate structures as hyphae, haustoria, and parenchyma were regularly restored to normally appearing turgor. Long-term storage of dried specimens (one for 84 yr; another 100 yr) was not at all detrimental to restoration.

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