Effect of recombinant human interleukin-6 on nitrite production of mouse myeloid leukemia cells

Abstract
The effect of recombinant human interleukin‐6 (rhlL‐6) on induction of nitrite (NO ) production was investigated in a mouse myeloid leukemia cell line (M1) and a subclone (Mm1). NO was induced by rhlL‐6 (>50 U/ml) in these cell lines. Pretreatment with rhlL‐6 (100 U/ml) for 1 day synergistically enhanced the production of NO in Mm1 by LPS. Furthermore, pretreatment withl lL‐6 (100 U/ml) shortened the lag time of induction. These results indicate that lL‐6 is involved in the regulation of the NO production in macrophage‐like cells.